Restriction Digest

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1. For a double digest, make sure that both enzymes are compatible and that a buffer exists in which both of them can work.
   
   
2. Add 6 uL of water into an empty Eppendorf tube.
   
   
3. Add 1uL of the buffer and 2 uL of the DNA or plasmid.
   
   
4. Add 0.5uL of each enzyme solution. Make sure the restriction enzymes are kept on ice before use.
   
   
5. Incubate for 1-2h at 37°C.